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Sartorius AG
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Thermo Fisher
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Image Search Results
Journal: Communications Biology
Article Title: Cystathionine β-synthase as novel endogenous regulator of lymphangiogenesis via modulating VEGF receptor 2 and 3
doi: 10.1038/s42003-022-03923-7
Figure Lengend Snippet: a Effect of various doses of AOAA on the proliferation efficiency of HDLECs after 24 h ( n = 5). Proliferation was determined by using IncuCyte Zoom. b Quantification of flow cytometrically detected Annexin V-positive cells 24 h after treatment with the indicated concentrations of AOAA and representative flow cytometry images ( n = 5). c AOAA treatment of HDLECs slowed wound closure compared to control cells in wound healing assay ( n = 4). d Effect of 1 mM and 4 mM AOAA treatment on HDLECs on tube formation. Evaluation was performed after 4 h and 24 h ( n = 5). Data are presented as means ± SEM. Statistical significance was analyzed with ( a , b ) one-way ANOVA and Dunnett’s multiple comparison test or ( c , d ) two-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01; *** p < 0.001; **** p < 0.0001.
Article Snippet: For statistical analysis, the fold increase in density within 24 h was calculated using the
Techniques: Flow Cytometry, Control, Wound Healing Assay, Comparison
Journal: Communications Biology
Article Title: Cystathionine β-synthase as novel endogenous regulator of lymphangiogenesis via modulating VEGF receptor 2 and 3
doi: 10.1038/s42003-022-03923-7
Figure Lengend Snippet: a Efficiency of CBS knockdown determined qRT-PCR 72 h post-transfection. Reduced expression of CBS in HDLECs after 72 h transfected with either siR_CBS-5, siR_CBS-6, or negative control (NC) siRNA ( n = 21). b Quantification of immunofluorescence staining of HDLECs transfected with either siR_CBS-5, siR_CBS-6, or negative control (NC) siRNA stained for CBS ( n = 3). c Effect of CBS silencing on HDLECs proliferation transfected with either siR_CBS-5 or siR_CBS-6 compared with NC siRNA transfected cells. Proliferation was determined 72 h after transfection by using IncuCyte Zoom ( n = 5). d Quantification of flow cytometrically detected Annexin V-positive HDLECs 72 h after transfection with either siR_CBS-5, siR_CBS-6, or negative control (NC) siRNA ( n = 5). e Wound healing assay with negative control (NC) siRNA, siR_CBS-5 or siR_CBS-6 siRNA treated HDLECs after 72 h transfection ( n = 6). f Tube formation assay after 72 h transfection of HDLECs transfected with either negative control (NC) siRNA, siR_CBS-5 or siR_CBS-6 siRNA. Evaluation was performed after 4 h and 24 h ( n = 4). Data are presented as means ± SEM. Statistical significance was analyzed with ( a – d ) one-way ANOVA and Dunnett’s multiple comparison test or ( e , f ) two-way ANOVA and Tukey’s multiple comparison test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
Article Snippet: For statistical analysis, the fold increase in density within 24 h was calculated using the
Techniques: Knockdown, Quantitative RT-PCR, Transfection, Expressing, Negative Control, Immunofluorescence, Staining, Wound Healing Assay, Tube Formation Assay, Comparison